Skip to main content
. 2011 Feb 22;19(6):1090–1096. doi: 10.1038/mt.2011.17

Figure 4.

Figure 4

Monitoring of extrinsic apoptosis and validation of Gluc secretion. (a) GFP-DEVD-ssGluc as a biosensor for extrinsic apoptosis. Gli36 cells-expressing GFP-DEVD-ssGluc were plated in a 96-well plate and treated with different concentrations of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in triplicates. Sixteen hours later, aliquots of conditioned medium (in triplicates) were assayed for Gluc activity. (b–c) Cleaved ssGluc is secreted from cells via conventional secretory pathway. Gli36 cells-expressing GFP-DEVD-ssGluc or native Gluc were treated with phosphate-buffered saline (PBS) or brefeldin A (BFA; 5 µg/ml) for 2 hours. Cells were then treated with either PBS or doxorubicin (0.8 µmol/l) in triplicates and, 8 hours later, conditioned media and lysates were assayed for Gluc activity. Data presented as mean ± SD for relative Gluc activity in which the signal obtained from control native ssGluc treated with PBS only in b or treated with dox only (no BFA) in c is set to 1.