Fig. 1.

SSCP gel analysis of cadherin-focused RT-PCR reactions. RT-PCR reactions were run as described in materials and methods. ³³P-radiolabeled PCR products were run on a 4–10% polyacrylamide gradient gel, and results were developed on X-ray film. Lane 1, molecular weight markers; lane 2, PCR products from reaction using RNA from Madin-Darby canine kidney (MDCK) cells; lane 3, PCR products resultant from RNA isolated from normal kidney (NK) cells; lane 4, PCR products using RNA isolated from ADPKD cyst epithelial cells. Arrows point to regions on the gels where predominat RT-PCR products are identified. Note that the bands are less defined and smeared in lanes 3 and 4 as compared with lane 2. This suggests that there are more diverse PCR products obtained from NK and ADPKD cells as compared with MDCK cells.