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. 2011 Apr 26;301(1):E187–E195. doi: 10.1152/ajpendo.00056.2011

Fig. 4.

Fig. 4.

Effect of consumption of a HF diet on leptin signaling in the arcuate nucleus. A and B: Western blot of pSTAT3 expression in the arcuate nucleus of LF, DR, and DIO rats after 8 wk on respective diets following administration of either saline (400 μl ip) or leptin (80 μg/kg ip). Administration of leptin increased pSTAT3 in nodose ganglia from LF and DR rats but not DIO rats. C: quantification of Western blots demonstrating that leptin increased STAT3 phosphorylation compared with saline in LF (P < 0.01), DR (P < 0.05), and DIO (P < 0.01) rats; n = 3 rats/group. D: phosphorylated STAT5, p42/44, and Akt in the arcuate nucleus of LF, DR, and DIO rats after 8 wk on respective diets following leptin injection (80 μg/ml ip). E: quantification of Western blot demonstrating that leptin signaling is not disturbed in LF, DR, and DIO rats fed respective diets for 8 wk; n = 3. a,bSignificant differences with different letters.