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. 2011 Apr 5;301(1):E40–E48. doi: 10.1152/ajpendo.00065.2011

Fig. 3.

Fig. 3.

Inhibition of DOR reduces TH stimulation of ALP activity in MC3T3-E1 cells. A: expression of DOR in MC3T3-E1 cells transduced with scramble control short-hairpin RNA (shRNA) or DOR shRNA as determined by real-time RT-PCR analysis. Values are presented as means ± SE (n = 4). AP < 0.01 vs. scramble control. B: DOR protein level as determined by Western blot using whole cell lysates. Scramble control and DOR shRNA MC3T3-E1 cells were treated with T3 (0.1, 1, 3, and 10 ng/ml; C) or T4 (10, 100, and 1,000 ng/ml; D), and ALP activity was determined 72 h after treatment. Values are presented as %vehicle-treated scramble control ± SE (n = 6–8). Basal ALP activity values of scramble control (0.35 mU/mg protein ± 0.01) and DOR shRNA (0.23 mU/mg protein ± 0.01). Data were analyzed by 2-way ANOVA, and a significant interaction was observed between gene and treatment. AP < 0.05 vs. vehicle-treated control; BP < 0.05 vs. scramble control at corresponding dose.