Fig. 2.

Effect of mitochondrial inhibitors on PMET activity. INS-1 832/13 cells (96-well plates) and mouse islets (20 islets/0.5-ml tube) were preincubated for 2 h in 2 or 4 mM glucose, respectively. PMET activity was measured by the formation of WST-1-formazan (A and B) or ferrocyanide (C and D). *P < 0.05 compared with the corresponding control value. Rotenone and antimycin were applied at 10 μM, aminooxyacetate (AOA) at 1 mM, and KCN at 0.5 mM final concentration. Data are means ± SE from 3–4 independent experiments performed in triplicate measurements.