Fig. 3.

Elevated protein synthesis and mammalian target of rapamycin (mTOR) signaling in skeletal muscle of R6/2 mice. A: elevated protein synthesis rate in gastrocnemius of 12-wk-old male mice. *P < 0.05, R6/2 vs. WT mice, n = 10/group. B: Western blot analysis of eIF4E-binding protein-1 (4E-BP1) in gastrocnemius of 12-wk-old male mice fasted for 6 h, and ratio of hyperphosphorylated γ-isoform to total 4E-BP1 and total 4E-BP1 level normalized by GAPDH are shown. Western blot analysis of mTOR phosphorylation and total mTOR (C), 4E-BP1 (D), and T389 S6K1 phosphorylation (E) in 12-wk-old male mice after 21-h fasting then 3-h refeeding. *P < 0.05, n = 8 for WT and 5 for R6/2.