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CRH peptide levels in anterior hypothalamus are increased by VGB treatment. Quantitative analysis of CRH peptide in the microdissected paraventricular and adjacent hypothalamic nulcei was achieved by using RIA. Rats were killed 24 h after saline or VGB treatment. Values are expressed as the mean CRH peptide content per rat, as obtained from five rats per group. Error bars represent the standard error of the mean. *Significantly different from saline controls (p < 0.05).
