Fig. 4.

Alterations of angioregulatory proteins in FVB/NJ background mice and MMP-9-null mice following injury. Kidneys from mice that underwent renal artery clamping for 20 min or sham operation were harvested 14 days following the procedure and processed for Western blotting. A: representative lanes from the same immunoblot of tissue-bound vascular endothelial growth factor (VEGF; VEGF₁₈₈, ∼24-kDa monomer) above the densitometric analysis of the pooled immunoblots for each group. B: representative lanes from the same immunoblot of cryptic extracellular matrix (ECM) peptides corresponding to arresten (∼26 kDa from the NC1 domain of the α-1 chain of collagen IV) and canstatin (∼25 kDa from the NC1 domain of the α-2 chain of collagen IV) above the densitometric analysis of the pooled immunoblots for each group. S, sham-operated; I, ischemia. Values are expressed as mean densities normalized to actin (SD). *P < 0.05 relative to S mice, n = 3.