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Representative immunoblots of COS7 cells transfected with NOS1α or DNM2-GFP constructs and coimmunoprecipitation with anti-NOS1. A: expression of transfected NOS1α in COS7 cells. B: GFP was only expressed in the DNM2-GFP-transfected cells. C: COS7 cell lysates IP with anti-NOS1 and IB with anti-GFP demonstrating NOS1 and DNM2 interact in vitro. The vehicle treatment was transfection agent fugene only.
