Fig. 2.

Effects of chronic NIC exposure on kidney morphology and integrity of cultured proximal tubule. Sections of kidneys form saccharine+sham (A), saccharine+IR (B), NIC+sham (C), and NIC+IR (D) were stained with PAS and evaluated for injury as described in materials and methods. TKPTS cells were treated or not with 200 μM NIC for 24 h followed by 200 μM H₂O₂ for 3 h and stained with calcein/ethidium as described in materials and methods. Pictures are representatives of 3 independent experiments. E: control, untreated. F: H₂O₂-treated; G: NIC alone; H: NIC+H₂O₂.