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. 2011 Apr 22;301(1):L40–L49. doi: 10.1152/ajplung.00299.2010

Fig. 2.

Fig. 2.

Transduced alveolar epithelial cells express YFP-Cldn-3 and YFP-Cldn-4 within a physiological range. A: immunoblot analysis using anti-green fluorescent protein antibodies showed comparable expression of YFP-Cldn-3 and YFP-Cldn-4 in cells collected on day 6. B and C: immunoblot analysis of samples using anti-Cldn-3 (B) or Cldn-4 (C) antibodies demonstrates the presence of the claudin-specific epitope for both constructs. Note relative difference in expression of endogenous Cldn-3 (B) and Cldn-4 (C). un, Untreated. D: use of YFP-tagged constructs enabled Cldn-3 and Cldn-4 protein content to be uniformly normalized relative to each other. Note ∼5-fold more endogenous Cldn-4 than endogenous Cldn-3 in alveolar epithelial cells on day 6. Transduction with any of the adenovector constructs had little effect on endogenous Cldn-3 or Cldn-4 compared with untreated (untr) cells. Values are means ± SE (n = 4–6).