Fig. 7.

Total endogenous tight junction protein content was not affected by increased expression of Cldn-3 or Cldn-4. A: primary rat alveolar epithelial cells were untreated (un) or transduced on day 4 with YFP-Cldn-3, YFP-Cldn-4, or YFP alone, further incubated for 2 days, and then subjected to immunoblot analysis for expression of Cldn-1, Cldn-5, Cldn-7, Cldn-18, occludin, ZO-1, and ZO-2. B: expression of YFP-Cldn-3 or YFP-Cldn-4 did not have a significant effect on expression of these endogenous tight junction proteins as measured by densitometric analysis of tight junction protein expression normalized using parallel samples analyzed for actin. Values are means ± SE (n = 3–4).