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. 2011 Apr 8;301(1):L117–L124. doi: 10.1152/ajplung.00417.2009

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Fig. 6. — Cytosolic cAMP does not phosphorylate membrane-localized filamin A. Infection of PMVECs with Pseudomonas aeruginosa ( P. aeruginosa) (multiplicity of infection 20:1) expressing ExoY (ExoY) (A) leads to an increase in tau phospho-serine 214, which is absent with the catalytically inactive ExoY strain (K81M). B: infection with P. aeruginosa (multiplicity of infection 20:1) expressing ExoY (ExoY) or K81M does not increase filamin 2152 phosphorylation. A and B: total filamin expression does not change, and tubulin or actin act as loading controls.