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Duolink Assay results for static and flow exposed HAEC. Protein associations determined by Cy3 channel confocal microscopy signal. Nuclei are counterstained with 4',6-diamidino-2-phenylindole (DAPI). A: total Akt and Smad2 association, n = 2. B: Smad2 and pAkt473 association. Enhanced cytoplasmic staining is evident with both sets of antibodies, indicating that colocalization of the proteins was occurring preferentially under flow stimulation.
