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Nuclear transforming growth interacting factor (TGIF) levels after 20 h of 2 and 10 dyn/cm2 average shear stress at 80 ± 5 mmHg, and exemplary Western blot lanes. Plotted data represent average relative amounts as determined by combining Western blot normalization versus same lane p53, α-tubulin, and backstained gel optical densities. For each case, n = 3. **P < 0.01.
