Fig. 1.

Localization of soluble guanylate cyclase-α₁ (sGCα₁) in cardiac myocytes (CM). CM isolated from wild-type (WT; A–C and G–I) and sGCα₁^−/− (D–F and J–L) mice were reacted with primary antibodies recognizing sGCα₁ (B and E), sGCβ₁ (H and K), and α-actinin (A, D, G, and J), and bound antibody was visualized by confocal microscopy using fluorescently labeled secondary antibodies. α-Actinin was detected with FITC-labeled anti-mouse IgG (green), and sGCα₁ and sGCβ₁ were detected using Dylight 594-labeled anti-rabbit IgG (red). Merged view (C, F, I, and L) demonstrates that sGCα₁ colocalizes with α-actinin on Z-lines. Scale bars (white) = 10 μm.