Fig. 1.

Doxycycline (DOX) treatment reduced the expression of active-matrix metalloprotease-2 (MMP-2) in the aorta of SHRSP. Aliquots of aorta supernatants containing 50 μg of protein were resolved by SDS-PAGE and transferred to a PVDF membrane for Western blot analysis. DOX treatment reduced the expression of active-MMP-2, but not pro-MMP-2, in the aorta of stroke-prone spontaneously hypertensive rats (SHRSP; A). Data are shown as a ratio of MMP-2 expression to β-tubulin expression (loading control). B: representative images of blots. *P < 0.05, Student's t-test. Number of animals: SHRSP = 4, SHRSP + DOX = 4.