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. 2011 Apr 7;301(1):G91–G99. doi: 10.1152/ajpgi.00471.2010

Fig. 1.

Fig. 1.

Necrotic cell supernatant (NCS) stimulation of EPC2-hTERT cells leads to the inducible expression of IL-8. EPC2-hTERT cells were suspended in fresh keratinocyte serum-free medium (KSFM), subjected to 5 cycles of freeze-thaw necrosis, then centrifuged and separated into NCS and necrotic cell pellets (NCP). Fresh medium (control) and freeze-thawed medium (FT-KSFM) were used as controls. A: time course of IL-8 mRNA expression by live EPC2-hTERT monolayers following stimulation with NCS for various time points. B: quantification of IL-8 protein in medium (KSFM), freeze-thawed KSFM (FT-KSFM), and NCS before (fresh) and after (stimulated) being used to stimulate EPC2-hTERT cells for 3 h. C: expression of IL-8 mRNA by EPC2-hTERT cells following stimulation with fresh medium (control), FT-KSFM, NCP, and NCS at the 3-h time point. D: IL-8 mRNA expression in EPC2-hTERT cells following stimulation with neutral HBSS (control), acidified HBSS (acid-HBSS), and NCS made from acid-killed EPC2-hTERT cells (acid-NCS). Results are representative of 3 separate experiments. *P < 0.05, **P < 0.005, ***P < 0.001; NS, not significant.