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. 2011 Apr 7;301(1):G91–G99. doi: 10.1152/ajpgi.00471.2010

Fig. 3.

Fig. 3.

Reduced responses to self-derived NCS by transformed gastrointestinal epithelial cell lines. Esophageal TE-1 squamous cell carcinoma cells and Caco-2 colon cancer cells were subjected to 5 cycles of freeze-thaw necrosis. A: IL-8 expression by live TE-1 and Caco-2 monolayers following stimulation with self-derived NCS for 3 h. TE-1- and Caco-2-derived NCS was generated in DMEM cell culture medium. B: IL-8 expression by EPC2-hTERT monolayers following stimulation with NCS derived from TE-1 and Caco-2 cells. TE-1 and Caco-2 NCS was generated in KSFM. Freeze-thawed KSFM was used as a control. Results are representative of 3 separate experiments. *P < 0.05, ***P < 0.001.