Fig. 3.

Niemann-Pick C1-like 1 (NPC1L1) mRNA and protein expression is reduced by curcumin. A: Caco-2 cells were incubated for 24 or 48 h with 50 μM of curcumin, and total RNA was extracted. The relative expression of NPC1L1 mRNA normalized to actin mRNA was assessed by real-time RT-PCR. Data are presented as %control and represent the mean ± SE of 6–8 values obtained from at least 3 separate occasions. *P < 0.05 or less compared with control. B: Caco-2 monolayers were incubated with 50 μM curcumin for 48 h, and proteins were then extracted. Equal amounts of protein were separated on a 6% SDS-PAGE and electrotransferred to nitrocellulose membranes. Blots were then probed with anti-NPC1L1 or anti-actin antibodies, and bands were visualized as described in materials and methods. A representative of 3 different experiments is shown.