Figure 4. Inhibition of complement-mediated lysis of ER by rTs-Pmy.
Various amounts of rTs-Pmy (0, 10, 20 or 40 µg) were incubated with fresh NGS(6%), and then fresh ER were added (3×108 cells). The amount of hemoglobin released into the supernatant was measured at 412 nm, and the percent lysis was calculated. The results are shown as the means ± SD for three independent experiments.