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. 2011 Jul 5;5(7):e1225. doi: 10.1371/journal.pntd.0001225

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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C9 (3 µg) was pre-mixed with various amounts of rTs-Pmy (Lane 2, 0 µg; Lane 6, 10 µg; Lane 7, 2.5 µg and Lane 8, 1 µg) or rTs87 (Lane 3, 10 µg; Lane 4, 2.5 µg) and incubated with 50 µM ZnCl₂. Lane 5, blank C9 without adding Zn²⁺ as a negative control. Lane 1, standard protein marker. The reaction mixtures were analyzed by SDS-PAGE and Western blot with anti-C9 mAb for Lane 8 as shown in Lane 9.