Table 1. Relative Levels of Representative Lipids Measured by Metabolite Profiling in Samples with HL∶VLDL and GFP∶VLDLa.
Lipid Class | HL∶VLDL/GFP∶VLDL |
Phospholipids | |
C34:1 PC | 0.83 |
C34:2 PC | 0.73* |
C36:2 PC | 0.70* |
C36:3 PC | 0.66* |
TAGs | |
C48:2 | 0.34* |
C50:2 | 0.40* |
C54:6 | 0.34* |
DAGs | |
34:1 | 0.10* |
36:2 | 0.09* |
LPCs | |
C18:1 | 4.2* |
CEs | |
C18:1 | 0.97 |
C18:2 | 0.52 |
SM | |
C16:0 | 0.81 |
C16:1 | 0.89 |
FFAs | |
C16:0 | (2.1)* |
C16:1 | (7.7)* |
C18:1 | (4.6)* |
C20:4 | (6.1)* |
C20:5 | (7.0)* |
C22:6 | (1.1) |
Data in parentheses were determined by directly quantifying FA levels using targeted LC/MS method with internal FA standard. TAGs, triacylglycerols; DAGs, diacylglycerols; LPCs, lysophosphatidic acid; CEs, cholesterol esters; SM, sphingomyelin; FFAs, free fatty acids. Data represent the mass ion intensity ratios performed in triplicate for each experimental condition.
*p<.05 for HL∶VLDL compared to GFP∶VLDL.