Figure 1. ING5 interacts with INCA1 in vitro and in vivo.

(A) In GST pull-down assays, GST alone or GST fused to INCA1, were incubated with [³⁵S] labeled genes which were selected through a yeast two-hybrid (Y2H) screening. Nine known genes were confirmed to interact with INCA1 in vitro. (B) COS-7 cells were transfected with EGFP-INCA1 and ING5. Immunoprecipitation with anti-EGFP antibody and subsequent Western blotting for ING5 demonstrated the in vivo interaction of ING5 and INCA1. (C) Immunoprecipitation with anti-ING5 antibody and subsequent Western blotting for EGFP-INCA1 confirmed the in vivo interaction. (D) Ing5 gene expression was decreased in AML specimens as determined by quantitative real-time RT-PCR assays based on Taqman technology. AML specimens were obtained at the time of diagnosis (p = 0.02).