Figure 4. Pericardial cells are in excess and mislocalized in heph⁰³⁴²⁹ embryos indicating that cardiogenesis is disrupted in these embryos.

A–C. The same heph⁰³⁴²⁹ and wild type embryos imaged from different perspectives. D. Pericardin was present in unusual places in heph⁰³⁴²⁹ embryos, such as the head and the tail. Note the large amnioserosal region (as) that persisted in heph⁰³⁴²⁹ embryos. E. The ventral epidermis was reasonably developed in heph⁰³⁴²⁹ embryos indicating that these embryos produced sufficient amounts of canonical Notch signaling in the ventral region. F. Pericardial cells continued to be in excess and cardiogenesis blocked in heph⁰³⁴²⁹ embryos at the time when embryogenesis and dorsal vessel formation was complete in wild type embryos (stage 17). All embryos shown were from the same experiment and were processed identically.