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. Author manuscript; available in PMC: 2011 Jul 5.
Published in final edited form as: J Biol Chem. 2007 Jan 3;282(9):6242–6254. doi: 10.1074/jbc.M611178200

FIGURE 1. Production of several virulence factors in H. pylori (A) and translocation and phosphorylation of CagA (B).

FIGURE 1

Total lysates of different H. pylori and their corresponding ΔalpAB mutants were separated on 8–12% SDS gels, blotted to nitrocellulose, and incubated with specific antibodies that recognize various H. pylori virulence factors (A). Total lysates of AGS cells co-cultured with H. pylori were separated on 8% SDS gels, blotted to nitrocellulose, and incubated with the phosphotyrosine-specific monoclonal antibody PY99 (B). Typical gels for four parental strains and their ΔalpAB mutants (clone 1) are presented.