Skip to main content
. Author manuscript; available in PMC: 2011 Jul 5.
Published in final edited form as: J Biol Chem. 2007 Jan 3;282(9):6242–6254. doi: 10.1074/jbc.M611178200

FIGURE 3. IL-6 and IL-8 promoter activation following H. pylori infection.

FIGURE 3

Luciferase reporter activity of a plasmid containing a −162/+44 fragment of the IL-8 promoter and a plasmid containing the full-size human IL-6 promoter was measured. Three independent transfections, each done in triplicate, were performed. MKN28 cells were transiently transfected with 2 μg of plasmid containing IL-8 and IL-6 promoters and 10 ng of Renilla plasmid as an internal control. The cells were then infected with H. pylori for 9 h. Untreated cells served as controls. Normalized luciferase activity induced by H. pylori infection is expressed as -fold increase in H. pylori-infected cells relative to uninfected controls. Data are expressed as mean (left) and mean ± S.E. (right). *, p < 0.01 compared with WT strains. NS, not significant.