Table 1.

Model parameters for which all results (except Fig. 4C) are calculated unless otherwise stated

Parameter	Value	Description
Km	505.43 mM	The binding constant of Transporter M
Kx	500 μM	The binding constant of Transporter X [27]
K1	4.3 μM	The binding constant of Pmc1 [48]
K2	0.1 μM	The binding constant of Pmr1 [44]
K3	25 μM	The binding constant of Vcx1 [35]
KIM	149.18 mM	The Mg2+ inhibition constant of Transporter M
KIX	3.51 × 10−6 μM	The Mg2+ inhibition constant of Transporter X
Vm	1.239 × 10−16 mol min−1	The rate parameter of Transporter M
Vx	3.2 × 10−17 mol min−1	The rate parameter of Transporter X (calculated from [27])
V1	4.459 × 10−17 mol min−1	The rate parameter of Pmc1
V2	4.394 × 10−17 mol min−1	The rate parameter of Pmr1
V3	1.682 × 10−15 mol min−1	The rate parameter of Vcx1
ka	0.0448	The feedback control constant of Transporter M
kb	4.319	The feedback control constant of Transporter X
$k_{\text{M}}^{+}$	3.751 (μM)−3 min−1	The forward rate constant of Eq. (1)
$k_{\text{M}}^{-}$	0.02445 min−1	The backward rate constant of Eq. (1)
[CaMtotal ]	25 μM	The total concentration of calmodulin [12]
Lmax	1.9996 × 109 RLUs	The maximal aequorin luminescence
f	0.007626	The calcium buffer effect constant
Lp	6 × 10−12 m s−1 Pa−1	The hydraulic membrane permeability [29]
σ	0.035	The reflection coefficient
b	0.4V0	The non-osmotic volume of yeast cell [20]
ns	3.82 × 10−14 mol	The apparent number of osmotically active molecules in the cell (calculated from [20])
Π0	0.24 Osm	The initial osmotic pressure of the external medium [20]
V0	100 μm3	The initial volume of the yeast cell [20]