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. 2001 Apr 1;29(7):e40. doi: 10.1093/nar/29.7.e40

Figure 1.

Figure 1

Transgene activation strategy mediated by FLP recombinase. (A) Structure of the cassette plasmid, pCAFNF5, possessing the CAFNF target unit. CAG, CAG promoter; SpA, SV40 early poly(A) signal; MCS, multi-cloning site; GpA, rabbit β-globin poly(A) signal. The stuffer region is composed of the neo gene and the SpA flanked by a pair of FRT. (B) Structure of the FLP-expressing rAd, AxCAFLP, and the target rAds, AxCAFNFG and AxCAFNFZ. A triangle under the adenovirus genome represents the deletion of an adenovirus sequence. (C) Activation of the transgene by CAFNF target unit mediated by FLP recombinase. The horizontal arrows indicate the orientation of the transcription. FLP-mediated excisional deletion removes the stuffer region, resulting in the expression of the reporter genes, GFP or LacZ, under the control of the CAG promoter.