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. 2001 Apr 1;29(7):e40. doi: 10.1093/nar/29.7.e40

Figure 2.

Figure 2

In vitro recombination of the FLP/FRT system at 30°C. (A) Quantitation of the recombination products. M (lane 1), 1 kb DNA ladder marker (Gibco BRL); P and S, product and substrate, respectively. Quantitation standards (lanes 2–9) represent the NcoI fragment of the EcoRI-digested pCALNLZ. HindIII-linearized pCAFNFG and each denoted amount of the FLP lysate was incubated for 30 min at 30°C. The recovered DNA was then digested with FspI and the density of the recombined 3.9 kb products (P3.9) were quantified based on densities of the quantitation standards. One representative result out of three is shown. (B) Recombination process of the FLP/FRT system. F, FspI site.