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. 2001 Apr 1;29(7):e40. doi: 10.1093/nar/29.7.e40

Figure 3.

Figure 3

In vitro recombination of the Cre/loxP system at 37°C. (A) Quantitation of the recombination products. M (lane 1), 1 kb DNA ladder marker (Gibco BRL); P and S, product and substrate, respectively; i, recombination intermediate; *, unexpected bands (may be due to the site preference of NcoI). The same quantitation standards (lanes 2–9) as used in the FLP/FRT system were applied. EcoRI-linearized pCALNLZ and each denoted amount of the Cre lysate was incubated for 30 min at 37°C. The recovered DNA was then digested with NcoI and the densities of the 4.4 kb products (P4.4) were quantified based on those of the quantitation standards. One representative result out of three is shown. (B) Recombination process of the Cre/loxP system. N, NcoI site.