Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Recombination efficiency of FLP and Cre in vitro. The recombination efficiency per lysate of FLP at 37°C (filled circle) or 30°C (open circle) and that of Cre at 37°C (filled square) or 30°C (open square) were quantified as described. The maximum amount of the reacted lysate was 25 µl in our experimental condition of 50 µl total reaction volume. The quantitative results were normally reproducible within ±5%. One representative result out of three is shown.
