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. Author manuscript; available in PMC: 2011 Jul 6.
Published in final edited form as: Biochem J. 2011 Apr 15;435(2):529–537. doi: 10.1042/BJ20100773

Figure 2. KLF11 inhibits proliferation, anchorage-independent growth and tumor formation in vivo in pancreatic cancer cells.

Figure 2

a. KLF11 inhibits cell proliferation in pancreatic cell lines. As a measurement of proliferation, the rate of BrdU incorporation was assessed in AsPC1, Capan2, CFPAC, L3.6 and MiaPaCa2 with a control or KLF11- adenovirus. Cell proliferation was significantly inhibited in KLF11-infected cells compared with control-infected cells (p<0.05). b. KLF11 decreases anchorage-independent growth. KLF11 infection of Capan2, L3.6, MiaPaCa2 and PANC1 cell lines resulted in substantial reduction of colony formation in soft agar compared to control (p<0.05). c. KLF11 inhibits pancreatic tumorigenesis in vivo. KLF11- or control-infected Capan2 and L3.6 cells were injected subcutaneously into the flanks of athymic nude mice and tumor volume was monitored. In vivo tumor formation was significantly reduced in mice injected with tumor cells expressing KLF11 as compared to control mice (p<0.05). A representative example of tumor mass is shown. Bottom panel shows western blot results of lysates from the pool of injected cells to confirm KLF11 expression. d. Graphical representation of the mean tumor volume after 6 weeks is shown, indicating that KLF11 inhibits the transformed phenotype of pancreatic cells in vivo (p<0.05) to support its role as a tumor suppressor in pancreatic cancer.