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. 2010 Oct 25;62(10):3359–3374. doi: 10.1093/jxb/erq321

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 3. — BvFLK complements the A. thaliana flk-1 mutant. (A) Phenotypes at 51 d after sowing of the A. thaliana flk-1 mutant, the ecotype Col-0, and the flk-1 mutant transformed with BvFLK driven by the CaMV 35S promoter (35S::BvFLK), BvFLK driven by the endogenous promoter of BvFLK in sugar beet (endo::BvFLK), or A. thaliana FLK driven by the CaMV 35S promoter (35S::AtFLK) (T₁ plants). Plants were grown under long-day conditions. (B) RT-qPCR expression analysis of FLC in flk-1, Col-0, and transgenic T₃ plants carrying the 35S::BvFLK, endo::BvFLK, or 35S::AtFLK transgene in the flk-1 mutant background. For each of the transgenic lines, two T₃ plants were tested that were derived from different transgenic individuals of a T₂ family. (C) RT-qPCR expression analysis of FLK in flk-1, Col-0, and transgenic 35S::AtFLK T3 plants. Expression levels in B and C were normalized against GAPDH and measured in triplicate. Error bars indicate the standard deviations of the mean. (This figure is available in colour at JXB online.)