Table 2.
Number of days to bolting (DTB) and total number of leaves at bolting (TNL) of primary transformants (T1 generation) and transgenic plants in segregating T2 populations derived from transformation of BvFLK and FLK into A. thaliana Col-0 and the flk mutant SALK_112850 (flk-1)
| Overexpression in Col-0 |
Complementation in flk-1 |
|||||
| Genotype | No. of plants | DTB (mean± SD) | TNL (mean ±SD) | No. of plants | DTB (mean ±SD) | TNL (mean ±SD) |
| T1 generation | ||||||
| 35S::BvFLK | 32 | 29.59±2.43 a | 10.59±1.83 a | 25 | 32.00±2.10 a | 13.08±1.55 a |
| endo::BvFLK | 15 | 30.00±2.56 a | 11.20±1.52 a | 9 | 34.89±3.79 a | 13.33±2.29 a |
| 35S::AtFLK | 10 | 30.90±2.08 a | 12.00±1.94 b | 4 | 31.50±4.20 a | 10.57±1.26 a |
| Col-0 | 16 | 35.69±1.70 b | 13.31±1.14 b | 16 | 35.69±1.70 a | 13.31±1.14 a |
| flk-1 mutant | – | – | – | 22 | 67.71±7.15 b | 66.35±1.32 b |
| F-value (probability) | 27.45 (0.00) | 10.03 (0.00) | 383.00 (0.00) | 3397.00 (0.00) | ||
| LSD0.05a | 2.14 | 1.46 | 4.42 | 2.05 | ||
| T2 generation | ||||||
| 35S::BvFLK | 13 | 27.77±2.59 a | 15.23±1.79 a | 14 | 34.14±3.06 a | 15.43±1.16 a |
| endo::BvFLK | 13 | 28.46±1.66 a | 15.31±1.71 a | 12 | 33.92±3.62 a | 15.51±1.29 a |
| 35S::AtFLK | 14 | 27.23±2.05 a | 15.00±2.00 a | 15 | 34.13±5.60 a | 15.80±1.08 a |
| Col-0 | 17 | 37.88±1.50 b | 17.53±1.01 b | 17 | 37.88±1.50 a | 17.53 ±1.01 a |
| flk-1mutant | – | – | – | 17 | 69.82±4.63 b | 67.82±2.01 b |
| F-value (probability) | 95.38 (0.00) | 8.34 (0.00) | 222.44 (0.00) | 2307.00 (0.00) | ||
| LSD0.05a | 1.85 | 1.41 | 4.06 | 1.86 | ||
a
Fisher's least significant difference at α=0.05. The letters a and b indicate significant differences between the mean values given in a table column (i.e. mean values in table cells including the letter ‘b’ are significantly different from the mean values in table cells including the letter ‘a’)