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. 2011 Feb 28;62(10):3433–3447. doi: 10.1093/jxb/err015

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© 2011 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 3. — Shoot proteome analysis. (A) Standard 2-DE map of proteins extracted from A. halleri shoots corresponding to the Mt+8S sample, with an average of 346±49 spots (Ctr and Mt samples showed an average of 562±21 and 377±11 spots, respectively). The 35 protein spots showing statistically significant differences between the conditions tested are marked by an open circle and numbered according to Table 4. (B) Close-up view of spots (indicated by arrows) representative of differentially expressed proteins (P <0.05) between control and treatments. (C, left) Validation of five differentially expressed proteins by Western blot (a representative gel of three repetition is reported). Ponceau-Red staining is shown for control of protein loading. (C, right) Densitometric analysis of immunoblot signals.