Table 4.
No | SSP | Protein name | AC number (gi NCBI) | MW (kDa)/pI theoretical | Peptide | Mascot | Fold variationsa | ||||
and reference organism | count | score | Mt+8S versus Ctr | Mt+8S versus Mt | |||||||
Photosynthesis, light reactions | |||||||||||
1 | 1204 | PSBP1b | At1g06680 | 28078/6.90 | 11 | 445 | ↑4.45 | ||||
Arabidopsis thaliana | |||||||||||
2 | 2204b | PSBP1b | At1g06680 | 20078/6.90 | 10 | 369 | ↑2.67 | ||||
Arabidopsis thaliana | |||||||||||
3 | 5101 | PSBP2 | At2g30790 | 27704/9.08 | 6 | 204 | ↓2.43 | ||||
Arabidopsis thaliana | |||||||||||
4 | 1302 | PSBO2b | At3g50820 | 34998/5.92 | 9 | 388 | ↑2.43 | ||||
Arabidopsis thaliana | |||||||||||
5 | 1303 | PSBO2b | At3g50820 | 34998/5.92 | 15 | 775 | ↑2.58 | ||||
Arabidopsis thaliana | |||||||||||
6 | 1311 | PSBO1-like | At4g37230 | 35114/5.68 | 17 | 672 | n.i.c | ||||
Arabidopsis thaliana | |||||||||||
7 | 2407 | HCF136 | At5g23120 | 44076/6.79 | 9 | 417 | ↑3.27 | ||||
Arabidopsis thaliana | |||||||||||
8 | 2103 | δ-subunit ATPase | At4g09650 | 25653/9.04 | 7 | 346 | ↑2.78 | ||||
Arabidopsis thaliana | |||||||||||
9 | 4004 | β-subunit ATPaseb | AtCg00480 | 52625/5.28 | 5 | 194 | ↑3.02 | ||||
Arabidopsis thaliana | |||||||||||
10 | 2601a | β-subunit ATPaseb | AtCg00480 | 52905/5.10 | 14 | 749 | ↑3.0 | ||||
Arabidopsis thaliana | |||||||||||
Photosynthesis, dark reactions | |||||||||||
11 | 1106 | Rubisco: large subunit | AtCg00490 | 52922/5.88 | 5 | 148 | ↑6.03 | ||||
Arabidopsis thaliana | |||||||||||
12 | 4611 | Rubisco: large subunit | ArhiCp028 | 52933/5.96 | 15 | 614 | ↑2.93 | ||||
Arabis hirsuta | |||||||||||
13 | 5704 | Rubisco: large subunit | ArhiCp028 | 52933/5.96 | 19 | 761 | ↑2.44 | ||||
Arabis hirsuta | |||||||||||
14 | 3008 | Rubisco: small subunit | At5g38430 | 20273/7.59 | 4 | 160 | ↑7.20 | ||||
Arabidopsis thaliana | |||||||||||
15 | 4001 | Rubisco: small subunit | gi|406727 | 20178/8.24 | 4 | 95 | ↑3.48 | ||||
Brassica napus | |||||||||||
16 | 8001 | Rubisco: small subunit | At1g67090 | 20448/7.59 | 6 | 253 | ↑2.41 | ||||
Arabidopsis thaliana | |||||||||||
17 | 6007 | Rubisco: small subunit | At5g38430 | 20273/7.59 | 5 | 223 | ↑2.04 | ||||
Arabidopsis thaliana | |||||||||||
18 | 1505 | Phosphoribulokinase | At1g32060 | 44436/5.71 | 13 | 475 | ↑2.16 | ↑2.13 | |||
Arabidopsis thaliana | |||||||||||
19 | 2503 | Rubisco activase | At2g39730 | 52006/5.69 | 10 | 446 | ↑2.4 | ||||
Arabidopsis thaliana | |||||||||||
Stress | |||||||||||
20 | 7507 | AtMDAR1b | At3g52880 | 46458/6.41 | 9 | 439 | ↓2.5 | ||||
Arabidopsis thaliana | |||||||||||
21 | 3504 | AtMDAR2b | At5g03630 | 47450/5.24 | 16 | 496 | ↓2 | ||||
Arabidopsis thaliana | |||||||||||
22 | 4203 | Fe-Superoxide | At4g25100 | 25409/6.30 | 3 | 165 | ↑2.16 | ||||
dismutase, FSD1 | Arabidopsis thaliana | ||||||||||
23 | 4101 | Allene oxide | At3g25770 | 21199/5.65 | 6 | 247 | ↓4.54 | ↓4.05 | |||
cyclase | Arabidopsis thaliana | ||||||||||
24 | 7307 | Endochitinase | gi|7798632 | 32004/5.93 | 5 | 156 | ↓5 | ||||
(Class I) | Arabis holboellii | ||||||||||
25 | 8005 | Endochitinase | gi|7798632 | 32004/5.93 | 3 | 133 | ↓3.85 | ||||
(Class I) | Arabis holboellii | ||||||||||
26 | 5003 | Malate dehydrogenase | At1g04410 | 35548/6.11 | 3 | 97 | ↑5.13 | ||||
Arabidopsis thaliana | |||||||||||
27 | 6101 | Germin-like protein | At5g20630 | 21849/6.81 | 2 | 72 | ↑2.44 | ↑2 | |||
AtGER3 | Arabidopsis thaliana | ||||||||||
28 | 5102 | Lipocalin, TIL1 | At5g58070 | 21421/5.97 | 2 | 84 | ↑2.65 | ||||
Arabidopsis thaliana | |||||||||||
29 | 2601b | Enolase | At2g36530 | 47689/5.54 | 9 | 433 | ↑3.0 | ||||
LOS2 | Arabidopsis thaliana | ||||||||||
Cellular metabolism and organization | |||||||||||
30 | 2204a | Plastidic chaperonin, | At5g20720 | 26785/8.86 | 11 | 572 | ↑2.67 | ||||
AtCPN20b | Arabidopsis thaliana | ||||||||||
31 | 5209 | Vegetative storage | At5g24770 | 29806/6.17 | 6 | 239 | ↓4.16 | ||||
protein, VSP2 | Arabidopsis thaliana | ||||||||||
32 | 5710 | α-subunit ATPase | AtMg01190 | 55011/6.23 | 20 | 1046 | ↓2.27 | ||||
mitochondrion | Arabidopsis thaliana | ||||||||||
33 | 6712 | β-glucosidase 1 | At1g52400 | 60462/6.89 | 11 | 383 | ↓6.25 | ↓2.48 | |||
Arabidopsis thaliana | |||||||||||
34 | 6713 | β-glucosidase 1 | At1g52400 | 60462/6.89 | 13 | 434 | ↓<10 | ||||
Arabidopsis thaliana | |||||||||||
35 | 7702 | β-glucosidase 1 | At1g52400 | 60462/6.89 | 7 | 228 | ↓3.45 | ||||
Arabidopsis thaliana |
Untreated control plants (Ctr); plants treated with 1 mM CdSO4 and 10 mM ZnSO4 (Mt); plants treated with 1 mM CdSO4 and 10 mM ZnSO4 and the addition of the eight selected bacterial strains (Mt+8S).
Fold variations in spot abundance between the different treatments and the corresponding control. No value in the table indicate that, for the corresponding spot, was not possible to identify a change that exceeds both a fold of variation >2 and a P <0.05.
Proteins whose expression pattern have been confirmed by Western analysis.
Newly induced.