Table 2.
Sequences of primer pairs specific to the A/G single nucleotide polymorphism of the SiDREB2 gene used in PCR and sizes and phenotypes of the PCR products generated by each primer pair
Allele | Primer sequence (5′–3′) | PCR product size | Phenotype |
Locus specific | SiDREB2 FL_F: CAACTCAGGAAGAAGAGGAT | 558 bp | Monomorphic |
SNP.TS_R: TGAAGTACCTCCACGGACTTGCACT | |||
DREB.AP2_F: ATGCATACGACGAGGCGGCAAAGG | 261 bp | Polymorphic | |
Allele specific | SNP.Tol_R: TTGAAGTACCTCCACGGACTTGCAC |