Fig. 3.

Effect of nitration on GAPDH. (A) Arabidopsis seedlings were treated with SIN-1. After the indicated times, the GAPDH activity levels were measured in crude protein extracts from whole seedlings as described in the Materials and methods. Measurements for activity were performed in triplicate and the average values ±SD are shown. (B) MS/MS spectrum of aminated LVSWYDNEWGYSSR peptide from Arabidopsis glyceraldehyde-3-phosphate dehydrogenase. Detected peaks of y and b series as well as immonium ions of L, Y, and W are indicated.