Table 3.
Structural features of potential Y targets of nitration in MALDI-TOF-identified proteins Protein annotation and AGI code along with the Putative nitrated Y are indicated. Parameters were calculated as described in the Materials and methods. Accessible solvent area (ASA) was calculated by NetSurfP software (Petersen et al. 2009).
| Protein/AGI | Putative nitrated Y | Distance to D/E | Proximal basic amino acids in primary sequence | Location in loop | ASA |
| Rubisco activase, chloroplast precursor_ At2g39730 | Y353 | (No model) | R351, R358, K359 | (No model) | 5.45 |
| Y76 | R72 | 79.13 | |||
| Serine hydroxymethyl transferase_ At4g13930 | Y167 | 5.99 Å to E342 | K160, K172, K176 | No | 62.38 |
| Y170 | 5.04 Å to D197 | K160, K172, K176 | No | 23.35 | |
| Transketolase, putative_ At3g60750 | Y337 | 9.01 Å to D268 | K333, H340, K347 | Yes | 66.16 |
| Glyceraldehyde-3-phosphate dehydrogenase, cytosolic_ At3g04120 | Y318 | 6.08 Å to D319 | K313, R327 | No | 7.35 |
| Y324 | 6.61 Å to E321 | K313, R327 | No | 19.17 | |
| Probable mannitol dehydrogenase_ At4g39330 | Y135 | 4.31 Å to E8 | K133, R147 | Yes | 34.66 |
| Y138 | 3.75 Å to D53 | K133, R147 | No | 13.55 | |
| Rubisco large subunit precursor_ AtCg00490 | Y239 | 6.33 Å to E158 | K236, H238, K252, R253 | No | 6.37 |