Table 4.
Genes involved in phenylpropanoid metabolism >2-fold differentially expressed between IN9 and IN13
| Family | Oligo ID | Signal intensity | Fold changea | Gene ID | Arabidopsis homologuegene | E-value | Putative annotation |
| ALDH | MZ00039837 | 10.5 | 5.5 | GRMZM2G071021 | AT3G24503 | 0E+00 | Cytosolic aldehyde dehydrogenase RF2C |
| CCR/DFR | MZ00000662 | 11.1 | –21.1 | gb|BT064982.1|b | AT1G15950 | 2E-125 | Cinnamoyl-CoA reductase/dihydroflavonol-4-reductase |
| CCR/DFR | MZ00000962 | 10.9 | –6.5 | GRMZM2G001991 | AT5G14700 | 7E-81 | Dihydroflavonol-4-reductase/cinnamoyl-CoA reductase-related |
| CCR/DFR | MZ00027625 | 11.5 | –5.8 | GRMZM2G050076 | AT2G33590 | 1E-58 | Dihydroflavonol-4-reductase/putative cinnamoyl-CoA reductase |
| CCR/DFR | MZ00034340 | 10.1 | 4.0 | GRMZM2G033555 | AT2G33590 | 2E-91 | Dihydroflavonol-4-reductase/putative cinnamoyl-CoA reductase |
| CCR/DFR | MZ00036789 | 11.3 | 4.0 | GRMZM2G034069 | AT2G33590 | 1E-100 | Dihydroflavonol-4-reductase/putative cinnamoyl-CoA reductase |
| CCR1 | MZ00015899 | 13.5 | –3.1 | GRMZM2G131205 | AT1G15950 | 2E-134 | Cinnamoyl CoA reductase1 |
| CCR/DFR | MZ00035958 | 9.4 | 2.8 | GRMZM2G034360 | AT2G33590 | 1E-100 | Dihydroflavonol-4-reductase |
| CCR/DFR | MZ00023228 | 9.9 | 2.4 | GRMZM2G057328 | AT1G15950 | 1E-103 | Dihydroflavonol-4-reductase |
| CCR/DFR | MZ00012815 | 9.2 | –2.3 | gb|BT036278.1|c | AT2G33590 | 2E-85 | Dihydroflavonol-4-reductase |
| CCR/DFR | MZ00030523 | 10.7 | –2.2 | GRMZM2G009681 | AT2G33590 | 4E-94 | Dihydroflavonol-4-reductase |
| OMT | MZ00044446 | 12.5 | 37.0 | GRMZM2G041866 | AT4G35160 | 4E-50 | O-Methyltransferase ZRP4-like |
| OMT | MZ00016324 | 11.7 | 14.7 | GRMZM2G140996 | AT4G35160 | 7E-53 | O-Methyltransferase ZRP4-like |
| OMT | MZ00042149 | 12.4 | –14.3 | GRMZM2G097297 | AT4G35160 | 2E-49 | O-Methyltransferase ZRP4 |
| OMT | MZ00026069 | 11.3 | 9.7 | ref|NM_001157182.1|c | AT4G35160 | 3E-25 | O-Methyltransferase ZRP4-like |
| OMT | MZ00042974 | 13.4 | 6.9 | GRMZM2G085924 | AT4G35160 | 1E-26 | O-Methyltransferase-like protein |
| FOMT | MZ00043408 | 15.1 | –4.2 | AC196475.3_FG004 | AT5G54160 | 3E-128 | Flavonoid O-methyltransferase |
| OMT | MZ00051351 | 9.7 | –2.2 | GRMZM2G141026 | AT4G35160 | 4E-52 | O-Methyltransferase ZRP4-like |
| CHI | MZ00036732 | 13.0 | –18.5 | GRMZM2G175076 | AT5G05270 | 1E-57 | Chalcone flavonone isomerase |
| CHI | MZ00026366 | 11.9 | –2.7 | GRMZM2G155329 | AT3G55120 | 6E-73 | Chalcone flavanone isomerase 1 |
| IFR | MZ00029320 | 14.0 | –6.4 | GRMZM2G326116 | AT1G32100 | 3E-122 | Isoflavone reductase-like protein |
| F3'H | MZ00019364 | 14.6 | –5.9 | gb|EU971853.1|b | AT5G07990 | 1E-65 | Cytochrome P450/flavonoid 3'-hydroxylase-like protein |
| F3'H | MZ00021196 | 10.7 | –5.5 | GRMZM2G146234 | AT5G24530 | 2E-61 | Flavanone 3-hydroxylase-like protein |
| F3'H | MZ00005460 | 11.2 | –5.4 | GRMZM2G160763 | AT5G07990 | 4E-161 | Cytochrome P450/ flavonoid 3-monooxygenase |
| CCoAOMT | MZ00000781 | 10.0 | –4.0 | GRMZM2G033952 | AT4G34050 | 5E-81 | Caffeoyl-CoA O-methyltransferase 1 |
| CCoAOMT | MZ00017952 | 11.8 | –2.4 | GRMZM2G004138 | AT4G34050 | 1E-80 | Caffeoyl-CoA O-methyltransferase 1 like |
| CCoAOMT | MZ00057269 | 14.0 | –2.0 | GRMZM2G099363 | AT4G34050 | 1E-112 | Caffeoyl-CoA O-methyltransferase 2 |
| PAL | MZ00014292 | 12.6 | –4.9 | GRMZM2G160541 | AT3G10340 | 0E+00 | Phenylalanine ammonia-lyase |
| PAL | MZ00039256 | 14.5 | –4.1 | GRMZM2G074604 | AT2G37040 | 0E+00 | Phenylalanine ammonia-lyase |
| PAL | MZ00025090 | 11.3 | –2.3 | GRMZM2G029048 | AT2G37040 | 0E+00 | Phenylalanine ammonia-lyase |
| PAL | MZ00034925 | 12.4 | –2.2 | GRMZM2G081582 | AT2G37040 | 0E+00 | Phenylalanine ammonia-lyase |
| 4CL | MZ00016350 | 13.1 | –3.8 | gb|BT067847.1|c | AT3G21240 | 0E+00 | 4-Coumarate coenzyme A ligase |
| 4CL | MZ00001892 | 10.4 | –3.0 | GRMZM2G019746 | AT5G63380 | 1E-135 | 4-Coumarate coenzyme A ligase family protein |
| 4CL | MZ00020111 | 10.1 | 2.9 | GRMZM2G054013 | AT1G65060 | 0E+00 | 4-Coumarate coenzyme A ligase, 4CL3 |
| 4CL | MZ00018351 | 10.4 | 2.1 | GRMZM2G080663 | AT5G63380 | 2E-70 | 4-Coumarate coenzyme A ligase family protein |
| Laccase | MZ00004658 | 9.8 | 4.8 | GRMZM2G132169 | AT5G05390 | 0E+00 | Laccase/L-ascorbate oxidase |
| Laccase | MZ00018473 | 10.2 | –4.1 | GRMZM2G031117 | AT5G05390 | 4E-147 | Laccase 1 |
| Laccase | MZ00004270 | 12.0 | –4.0 | GRMZM2G072780 | AT5G60020 | 0E+00 | Laccase |
| Laccase | MZ00055128 | 9.6 | –2.4 | GRMZM2G329311 | AT3G09220 | 1E-68 | Laccase |
| CAD | MZ00014812 | 13.9 | 2.4 | AC234163.1_FG004 | AT4G37980 | 6E-105 | Putative cinnamyl alcohol dehydrogenase |
| FLS | MZ00026581 | 11.7 | 3.3 | GRMZM2G152801 | AT5G08640 | 1E-101 | Flavonol synthase/flavanone 3-hydroxylase |
a
Positive values indicate fold higher expression in IN13 compared with IN9. Negative values indicate higher expression in IN9 compared with IN13.
b
THe oligo has too low homology using the maize genome browser.
c
No gene is associated with the oligo using the maize genome browser.
ALDH, aldehyde dehydrogenase; CAD, cinnamyl alcohol dehydrogenase, CCoAOMT, caffeoyl-CoA O-methyltransferase; CCR, cinnamoyl-CoA reductase; CHI, chalcone flavonone isomerase; 4CL, 4-coumarate:CoA ligase; DFR, dihydroflavonol-4-reductase; FLS, flavonol synthase; FOMT, flavonoid O-methyltransferase; F3'H, flavonoid 3'-hydroxylase; IFR, isoflavone reductase; OMT, O-methyltransferase; PAL, phenylalanine ammonia-lyase.