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. 2010 Dec 16;18(5):479–487. doi: 10.1038/gt.2010.163

Table 3. Effects of repeated infection on the transduction of CFC.

LV vectors Number of hits Number of CFC tested Vector-positive CFC (%) Median VCN within transduced CFC (range)
GFP-LV 1 78 56 1.4 (0.1–14.5)
  2 79 62 2.2 (0.1–20.9)
WASP-LV 1 151 29 0.9 (0.1–7.1)
  2 155 53 1 (0.1–6.5)

Abbreviations: CFC, colony-forming cell; GFP-LV, green fluorescence protein-lentiviral vector; VCN, vector copy numbers.

These experiments are also related in Figure 5 (see Figure legend). Vectors were tested at the concentration of 2 × 108 IG ml−1 (GFP-LV) or 0.5-1 × 108 IG ml−1 (WASP-LV) and data were pooled from two separate transductions with each vector.