Table 3. Effects of repeated infection on the transduction of CFC.
LV vectors | Number of hits | Number of CFC tested | Vector-positive CFC (%) | Median VCN within transduced CFC (range) |
---|---|---|---|---|
GFP-LV | 1 | 78 | 56 | 1.4 (0.1–14.5) |
2 | 79 | 62 | 2.2 (0.1–20.9) | |
WASP-LV | 1 | 151 | 29 | 0.9 (0.1–7.1) |
2 | 155 | 53 | 1 (0.1–6.5) |
Abbreviations: CFC, colony-forming cell; GFP-LV, green fluorescence protein-lentiviral vector; VCN, vector copy numbers.
These experiments are also related in Figure 5 (see Figure legend). Vectors were tested at the concentration of 2 × 108 IG ml−1 (GFP-LV) or 0.5-1 × 108 IG ml−1 (WASP-LV) and data were pooled from two separate transductions with each vector.