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. 2001 Apr 15;29(8):1741–1749. doi: 10.1093/nar/29.8.1741

Figure 4.

Figure 4

Figure 4

The U2/U6/BS crosslink contains U2/U6 helix II. (A and B) Unlabeled Adeno 5′ SS and 32P-labeled Adeno 3′ RNA were incubated in nuclear extract as in Figure 2A, and crosslinked as in Figure 2B. RNA was then purified, subjected to RNase H cleavage targeted by the indicated oligodeoxynucleotides, and the products resolved by denaturing 5% PAGE. The positions of the U2/BS and U2/U6/BS crosslinks, and the 3′ RNA substrate, are indicated. (A) Left to right, lanes 1–7. (B) Left to right, lanes 1–6.