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. 2011 Mar 2;39(12):5140–5148. doi: 10.1093/nar/gkr078

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Reactions with recombinase catalytic mutants reveal that HJ can be formed by either XerC or XerD mediated strand exchange at dif. Recombination reactions with combinations of proteins as indicated, were cut with EcoRI to reveal recombinant product and HJ. Many combinations tested produced some level of HJ indicating that either recombinases could potentially carry out the first pair of strand exchanges, even when FtsK_50C (K) or γ were present in the reaction. Reactions with FtsK_50C have a background smearing upon digestion, but this does not affect the interpretation. Proteins: C^YF= XerC^Y275F, C^KQ= XerC^K172Q, D^RK= XerD^R148K, D^KQ= XerD^K172Q.