Table 1.
Switch | EC50 (mM) | b = Y (0 mM) % | Y (5 mM) % | Fold-change | Dynamic range % | M % |
---|---|---|---|---|---|---|
RS | 0.54 ± 0.05 | 47 ± 2 | 91 ± 3 | 1.93 ± 0.10 | 44 ± 4 | 97 ± 1 |
RSN | 98 ± 3 | 101 ± 5 | 1.03 ± 0.06 | 3 ± 6 | ||
RSnt | 49 ± 2 | 59 ± 2 | 1.20 ± 0.07 | 10 ± 3 | ||
RS-theo2 | 1.47 ± 0.30 | 52 ± 2 | 84 ± 2 | 1.60 ± 0.06 | 32 ± 3 | 94 ± 3 |
RS-theo3 | 1.17 ± 0.09 | 41 ± 4 | 101 ± 4 | 2.47 ± 0.26 | 60 ± 6 | 116 ± 2 |
RS-B03 | 0.48 ± 0.08 | 79 ± 4 | 102 ± 4 | 1.29 ± 0.08 | 23 ± 5 | 105 ± 1 |
RS-B05 | 0.56 ± 0.07 | 51 ± 1 | 97 ± 4 | 1.91 ± 0.10 | 46 ± 4 | 104 ± 2 |
RS-B06 | 0.59 ± 0.11 | 56 ± 5 | 101 ± 6 | 1.82 ± 0.19 | 46 ± 7 | 108 ± 3 |
RS-B07 | 0.84 ± 0.04 | 44 ± 2 | 100 ± 4 | 2.30 ± 0.13 | 57 ± 5 | 110 ± 1 |
RS-B12 | 0.47 ± 0.08 | 44 ± 2 | 94 ± 4 | 2.11 ± 0.12 | 50 ± 4 | 99 ± 2 |
RS×2 | 1.07 ± 0.02 | 20 ± 1 | 79 ± 3 | 3.91 ± 0.19 | 59 ± 3 | 92 ± 0 |
RS×3 | 1.81 ± 0.06 | 10 ± 0 | 57 ± 1 | 5.57 ± 0.26 | 47 ± 1 | 74 ± 1 |
RS-B07×2 | 1.81 ± 0.11 | 16 ± 1 | 69 ± 4 | 4.24 ± 0.36 | 53 ± 4 | 89 ± 2 |
RS-B12×2 | 0.89 ± 0.07 | 22 ± 0 | 73 ± 1 | 3.30 ± 0.08 | 51 ± 1 | 81 ± 2 |
RS-theo3-B07 | 1.13 ± 0.10 | 37 ± 2 | 91 ± 2 | 2.47 ± 0.14 | 54 ± 3 | 103 ± 2 |
RS-theo3-B12 | 1.14 ± 0.13 | 36 ± 1 | 84 ± 1 | 2.31 ± 0.09 | 48 ± 2 | 95 ± 2 |
b and Y (5 mM) are experimentally determined values corresponding to the normalized protein levels at 0 mM and 5 mM theophylline, respectively. The EC50 and theoretical maximal output (M) are parameters determined by fitting the dose-response data to the binding model. The fold-change is the ratio of Y (5 mM) to b and the dynamic range is the difference between these two values. The theoretical fold-change and dynamic range determined by M instead of Y (5 mM) are reported in Supplementary Table S2.