Figure 3.
Analysis of photosynthesis in ycf3 intron deletion mutants and control plants. Data sets are shown for plants grown under high-light conditions (1000 μE m−2 s−1; left panels) and plants grown under low-light conditions (30 μE m−2 s−1; right panels). The values represent averages from five independent measurements/thylakoid isolations per plant line (± standard deviation). (A) Measurement of non-photochemical quenching (qN) in dependence on the light intensity. (B) Measurement of the photochemical quenching parameter qL. (C) Quantitation of the components of the photosynthetic electron transport chain by difference absorption spectroscopy. Data were subjected to one-way analysis of variance (ANOVA) using a pair-wise multiple comparison procedure (Holm–Sidak method) in SigmaPlot. Highly significant differences in PSI contents between the Δ intron 1 mutant and all other plants were observed in both high light and low light (P < 0.001; indicated by asterisks).