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Luciferase mRNA levels are not significantly affected by the uAUG insertions. Wild-type (wt) and mutant constructs were transfected into C33A cells, total RNA was isolated and subjected to primer extension reactions. cDNA products were analysed on a denaturing polyacrylamide gel (panel A) and quantified (panel B). The wt mRNA level was set at 1. A representative of two independent experiments is shown. The error bars indicate standard deviation.
