Figure 5.

Luciferase expression is similarly affected by the uAUG insertions in the presence or absence of HIV-1 particle production. C33A cells were transfected with wt or mutant luciferase construct in the absence or presence of 100 ng of the pLAI molecular clone. pRL-CMV was co-transfected as an internal control. The firefly and Renilla luciferase activities in the lysates were determined 2 days post-transfection. Firefly luciferase activity was corrected for Renilla luciferase activity. CA-p24 was determined in the supernatant and was consistent in all transfections (20.3 ng/ml average, 2.37 ng/ml standard deviation). The overall increase observed upon HIV-1 co- expression is similar as upon Tat co-expression (compare with Supplementary Figure S1). The relative representation of the data set is shown in Supplementary Figure S3. One of three independent experiments is shown, error bars indicate standard deviation.