UM1 cells were transfected with miR-7 mimic for 0, 24 or 48 h (left-hand panels). UM2 cells were treated with anti-miR-7 PNA for 0, 24 or 48 h (right-hand panels). The expression levels of IGF1R, IRS1, IRS2, PIK3CD, EGFR, RAF1 and PAK1 were examined by Western blotting as described in the Experimental section. Results shown are representative of at least three independent experiments. ACTB, β-actin.